| 1 |  Alessandra Keller (SWI) | | 0:03:43 |
| 2 | Linda Indergand (SWI) | | s.t. |
| 3 | Linda Zanetti (SWI) | Human Powered Health | 0:01 |
| 4 |  Nikola Bajgerová (CZE) | | 0:02 |
| 5 |  Kristen Faulkner (USA) | EF Education - Cannondale | s.t. |
| 6 | Jasmin Liechti (SWI) | | s.t. |
| 7 |  Lotta Henttala Lepistö (FIN) | | s.t. |
| 8 |  Katarzyna Wilkos (POL) | | s.t. |
| 9 | Jolanda Neff (SWI) | | 0:03 |
| 10 | Malwina Mul (POL) | | s.t. |
| 11 |  Nika Bobnar (SLO) | | 0:04 |
| 12 |  Clara Emond (CAN) | EF Education - Cannondale | s.t. |
| 13 |  Monica Trinca Colonel (ITA) | | s.t. |
| 14 |  Antonia Niedermaier (GER) | Canyon - Sram Racing | 0:05 |
| 15 | Olga Wankiewicz (POL) | | s.t. |
| 16 | Sina Frei (SWI) | | 0:06 |
| 17 | Clara Koppenburg (GER) | EF Education - Cannondale | s.t. |
| 18 |  Hanna Theys (BEL) | | s.t. |
| 19 | Lorena Leu (SWI) | Maxx-Solar Rose Women Racing | s.t. |
| 20 | Giorgia Bariani (ITA) | Top Girls Fassa Bortolo | s.t. |
| 21 | Magdeleine Vallieres Mill (CAN) | EF Education - Cannondale | s.t. |
| 22 |  Anastasiya Kolesava (BLR) | | 0:07 |
| 23 | Gabriela Bártová (CZE) | Team Dukla Praha Women | 0:08 |
| 24 | Lydia Ventker (GER) | Maxx-Solar Rose Women Racing | s.t. |
| 25 |  Ana Vitória Gouvea Vieira Almeida Magalhães (BRA) | | s.t. |
| 26 |  Nora Jencusova (SVK) | | s.t. |
| 27 |  Yanina Kuskova (UZB) | Tashkent City Women Professional Cycling Team | s.t. |
| 28 |  Victoire Joncheray (FRA) | Team Komugi - Grand Est | 0:09 |
| 29 |  Elisa Serné (NED) | | s.t. |
| 30 | Leonie Laubig (GER) | Primeau Vélo - Groupe Abadie | s.t. |
| 31 | Terézia Ciriaková (SVK) | | s.t. |
| 32 | Sara Pellegrini (ITA) | A.S.D. K2 Women Team | 0:10 |
| 33 | Patrycja Lorkowska (POL) | | s.t. |
| 34 | Gemma Sernissi (ITA) | A.S.D. K2 Women Team | s.t. |
| 35 |  Romina Hinojosa Cruz (MEX) | | s.t. |
| 36 | Skylar Schneider (USA) | | 0:11 |
| 37 | Zuzanna Chylinska (POL) | | s.t. |
| 38 | Gaia Tormena (ITA) | | s.t. |
| 39 | Asia Zontone (ITA) | | s.t. |
| 40 | Helena Bieber (GER) | Maxx-Solar Rose Women Racing | s.t. |
| 41 |  Tabea Huys (AUT) | Maxx-Solar Rose Women Racing | s.t. |
| 42 | Noemi Lucrezia Eremita (ITA) | A.S.D. K2 Women Team | 0:12 |
| 43 | Larissa Tschenett (SWI) | | s.t. |
| 44 | Laury Milette (CAN) | Team Komugi - Grand Est | s.t. |
| 45 | Ema Comte (FRA) | | s.t. |
| 46 | Sanne van Heel (NED) | | s.t. |
| 47 | Giada Borghesi (ITA) | | s.t. |
| 48 | Clémence Latimier (FRA) | | 0:13 |
| 49 | Anabelle Thomas (CAN) | Primeau Vélo - Groupe Abadie | s.t. |
| 50 | Valeria Curnis (ITA) | | s.t. |
| 51 |  Kimberly Cadzow (NZL) | EF Education - Cannondale | s.t. |
| 52 | Barbora Nemcová (CZE) | Team Dukla Praha Women | s.t. |
| 53 | Iris Monticolo (ITA) | Top Girls Fassa Bortolo | s.t. |
| 54 | Nicole Steinmetz (USA) | | 0:14 |
| 55 | Nikol Plosaj (POL) | | s.t. |
| 56 | Dziyana Lebedz (BLR) | | s.t. |
| 57 | Sandrine Etienne (FRA) | | s.t. |
| 58 | Alessia Missiaggia (ITA) | Top Girls Fassa Bortolo | s.t. |
| 59 | Iris Timmermans (NED) | | s.t. |
| 60 | Emanuela Zanetti (ITA) | | s.t. |
| 61 | Michela De Grandis (ITA) | | s.t. |
| 62 | Selene Colombi (ITA) | | s.t. |
| 63 |  Mille Troelsen (DEN) | | 0:15 |
| 64 | Julie Stockman (BEL) | | s.t. |
| 65 | Lea Fuchs (SWI) | Maxx-Solar Rose Women Racing | s.t. |
| 66 | Jony Van Den Eijnden (NED) | | s.t. |
| 67 | Veronika Bartoníková (CZE) | Team Dukla Praha Women | s.t. |
| 68 | Jule Märkl (GER) | | s.t. |
| 69 | Elisa Valtulini (ITA) | | 0:16 |
| 70 | Lara Crestanello (ITA) | | s.t. |
| 71 | Marie Vandecasteele (BEL) | | s.t. |
| 72 |  Miryan Maritza Nuñez Padilla (ECU) | Primeau Vélo - Groupe Abadie | s.t. |
| 73 | Kayla Davis (USA) | | s.t. |
| 74 | Tess Moerman (BEL) | | s.t. |
| 75 | Ilse Grit (NED) | | s.t. |
| 76 | Amber Lacompte (BEL) | | s.t. |
| 77 |  Awen Roberts (GBR) | | s.t. |
| 78 | Tess Edwards (USA) | | 0:17 |
| 79 | Nele Laing (GER) | Maxx-Solar Rose Women Racing | s.t. |
| 80 | Izabela Janusz (POL) | | s.t. |
| 81 | Jacqueline Tamez Escamilla (MEX) | | s.t. |
| 82 | Sara Piffer (ITA) | | s.t. |
| 83 | Carlotta Borello (ITA) | | s.t. |
| 84 | Silvia Magri (ITA) | Team Komugi - Grand Est | 0:18 |
| 85 | Marine Ferre (FRA) | | s.t. |
| 86 | Samantha Scott (USA) | | s.t. |
| 87 | Sonia Rossetti (ITA) | | s.t. |
| 88 | Natalia Krzeslak (POL) | | s.t. |
| 89 | Giulia Giuliani (ITA) | A.S.D. K2 Women Team | s.t. |
| 90 | Daniela Schmidsberger (AUT) | | s.t. |
| 91 | Claire Windsor (USA) | | s.t. |
| 92 | Janine Schneider (GER) | | 0:19 |
| 93 | Chiara Reghini (ITA) | Top Girls Fassa Bortolo | s.t. |
| 94 | Alphanie Midelet (FRA) | | s.t. |
| 95 | Nicole Hartychová (CZE) | Team Dukla Praha Women | s.t. |
| 96 | Spela Colnar (SLO) | | s.t. |
| 97 | Jarmila Machacová (CZE) | Team Dukla Praha Women | 0:20 |
| 98 | Romina Costantini (ITA) | | s.t. |
| 99 | Sara Mazzorana (ITA) | | s.t. |
| 100 | Ségolène Thomas (FRA) | Team Komugi - Grand Est | s.t. |
| 101 | Sandra Geyer (GER) | | s.t. |
| 102 | Karin Prinsen (NED) | | s.t. |
| 103 | Ursa Pintar (SLO) | | s.t. |
| 104 |  Daryna Nahuliak (UKR) | | s.t. |
| 105 | Gaia Segato (ITA) | Top Girls Fassa Bortolo | 0:21 |
| 106 | Jessie Marrel (FRA) | | s.t. |
| 107 | Margarita Misyurina (UZB) | Tashkent City Women Professional Cycling Team | s.t. |
| 108 | Martina Testa (ITA) | | s.t. |
| 109 | Bodine Vollering (NED) | | s.t. |
| 110 |  Yelizaveta Sklyarova (KAZ) | | 0:22 |
| 111 | Monika Brzezna (POL) | | s.t. |
| 112 | Elisa De Vallier (ITA) | Top Girls Fassa Bortolo | s.t. |
| 113 | Giulia Vallotto (ITA) | | s.t. |
| 114 | Maria Guadalupe Nava (MEX) | | s.t. |
| 115 | Emma Zanetti (ITA) | | s.t. |
| 116 | Sophie Brouckaert (BEL) | | s.t. |
| 117 | Katharina Becker (GER) | | 0:23 |
| 118 |  Argiro Milaki (GRE) | | s.t. |
| 119 | Lara Scarselli (ITA) | Aromitalia 3T Vaiano | s.t. |
| 120 | Alessia Foligno (ITA) | | s.t. |
| 121 |  Anabel María Fernanda Yapura Plaza (ARG) | Team Komugi - Grand Est | s.t. |
| 122 | Alice Papo (ITA) | A.S.D. K2 Women Team | s.t. |
| 123 | Sofiya Karimova (UZB) | Tashkent City Women Professional Cycling Team | s.t. |
| 124 |  Rasa Leleivyte (LTU) | Aromitalia 3T Vaiano | s.t. |
| 125 | Shaknoza Abdullaeva (UZB) | | s.t. |
| 126 |  Sharni Morley (AUS) | | 0:24 |
| 127 | Alice Capasso (ITA) | | s.t. |
| 128 | Olha Dehtiar (UKR) | | s.t. |
| 129 | Nina Schmidt (GER) | | s.t. |
| 130 | Jazmine Lavergne (CAN) | Primeau Vélo - Groupe Abadie | s.t. |
| 131 | Coralie Houde (CAN) | Primeau Vélo - Groupe Abadie | s.t. |
| 132 |  Demetra Koukouma (CYP) | Primeau Vélo - Groupe Abadie | 0:25 |
| 133 | Vania Cárdenas (MEX) | | s.t. |
| 134 | Fenna Hoegee (NED) | | s.t. |
| 135 |  Eyeru Tesfoam Gebru (ETH) | Team Komugi - Grand Est | 0:26 |
| 136 | Irene Affolati (ITA) | Aromitalia 3T Vaiano | s.t. |
| 137 | Giulia Miotto (ITA) | | s.t. |
| 138 | Nafosat Kozieva (UZB) | Tashkent City Women Professional Cycling Team | s.t. |
| 139 | Valeria Leon Ordaz (MEX) | | s.t. |
| 140 | Aurora Cappelletti (ITA) | | s.t. |
| 141 | Letizia Marzani (ITA) | | 0:27 |
| 142 | Alina Bogdan (UKR) | | s.t. |
| 143 | Kateryna Naberezhna (UKR) | | s.t. |
| 144 | Eliska Grulichová (CZE) | Team Dukla Praha Women | s.t. |
| 145 | Marie Lagershausen (GER) | | 0:28 |
| 146 | Camilla Lazzari (ITA) | | s.t. |
| 147 | Asal Rizaeva (UZB) | Tashkent City Women Professional Cycling Team | s.t. |
| 148 | Serena Brillante Romeo (ITA) | Aromitalia 3T Vaiano | s.t. |
| 149 | Kirstine Frida Rysbjerg Munk (DEN) | | 0:29 |
| 150 | Asia Ranucci (ITA) | | s.t. |
| 151 | Yuliia Pchelintseva (UKR) | | s.t. |
| 152 | Valeria Ponomarenko (UKR) | | s.t. |
| 153 | Eliza Rabazynska (POL) | | s.t. |
| 154 | Fanny Bonini (ITA) | Aromitalia 3T Vaiano | 0:30 |
| 155 | Monia Garavaglia (ITA) | | 0:31 |
| 156 | Alice Petrucci (ITA) | | s.t. |
| 157 | Monika Wegrzyn (POL) | | s.t. |
| 158 | Vittoria Ruffilli (ITA) | A.S.D. K2 Women Team | 0:32 |
| 159 | Martina Silvestri (ITA) | | 0:34 |
| 160 | Anita Poletti (ITA) | | s.t. |
| 161 | Livielle Ongarato (ITA) | | 0:35 |
| 162 | Viktoriia Parkhomiuk (UKR) | | s.t. |
| 163 | Marie Maes (BEL) | | 0:36 |
| 164 | Madina Kakhorova (UZB) | Tashkent City Women Professional Cycling Team | 0:37 |
| 165 | Daria Bilous (UKR) | | s.t. |
| 166 | Mayte Zamudio Garcia (MEX) | | 0:39 |
| 167 | Yuliia Nilova (UKR) | | 0:40 |
| 168 |  Fariba Hashimi (AFG) | | 0:41 |
| 169 | Beatrice Roda (ITA) | | 0:45 |
| 170 | Anastasiya Plotsidym (UKR) | | 0:46 |
| 171 | Valentina Trussardo (ITA) | | 0:54 |
