| 1 |  Alberto Contador Velasco (SPA) | Astana | 16:56:01 |
| 2 | Igor Antón Hernandez (SPA) | Euskaltel - Euskadi | 0:00:41 |
| 3 | Ezequiel Mosquera Miguez (SPA) | Xacobeo - Galicia | 0:01:20 |
| 4 |  Janez Brajkovic (SLO) | Radio Shack | 0:01:30 |
| 5 | Vicente David Bernabéu Armengol (SPA) | Barbot - Siper | 0:02:30 |
| 6 | Javier Moreno Bazan (SPA) | Andalucía - Cajasur | 0:02:51 |
| 7 |  Tiago Jose Pinto Machado (POR) | Radio Shack | s.t. |
| 8 |  Stefan Denifl (AUT) | Cervélo Testteam | 0:02:58 |
| 9 |  Denis Menchov (RUS) | Rabobank | 0:03:06 |
| 10 | José Luis Rubiera Vigil (SPA) | Radio Shack | 0:03:17 |
| 11 |  Koos Moerenhout (NED) | Rabobank | 0:03:23 |
| 12 | Raul Santamarta Valvuena (SPA) | Burgos 2016 - Castilla y Leon | 0:03:28 |
| 13 | Bruno Manuel Silva Pires (POR) | Barbot - Siper | 0:03:58 |
| 14 | Aketza Pena Iza (SPA) | Caja Rural | 0:04:02 |
| 15 | Gustavo César Veloso (SPA) | Xacobeo - Galicia | 0:04:20 |
| 16 |  Juan Mauricio Soler Hernandez (COL) | Caisse d'Epargne | 0:04:44 |
| 17 |  Grischa Niermann (GER) | Rabobank | 0:05:03 |
| 18 | José Herrada Lopez (SPA) | Caja Rural | 0:05:10 |
| 19 | Juan Manuel Gárate Cepa (SPA) | Rabobank | 0:05:11 |
| 20 |  Marzio Bruseghin (ITA) | Caisse d'Epargne | 0:05:15 |
| 21 | José João Pimenta Costa Mendes (POR) | LA - Rota Dos Moveis | 0:05:33 |
| 22 |  Fabricio Ferrari Barcelo (URU) | Caja Rural | 0:05:37 |
| 23 | Diego Gallego Arnaiz (SPA) | Burgos 2016 - Castilla y Leon | 0:07:33 |
| 24 | Alberto Losada Alguacil (SPA) | Caisse d'Epargne | 0:07:54 |
| 25 | Rubén Plaza Molina (SPA) | Caisse d'Epargne | 0:08:08 |
| 26 |  Andrey Amador Bikkazakova (CRC) | Caisse d'Epargne | 0:08:17 |
| 27 | Iñigo Cuesta Lopez De Castro (SPA) | Cervélo Testteam | 0:08:18 |
| 28 | Pablo Lastras Garcia (SPA) | Caisse d'Epargne | 0:08:22 |
| 29 | Daniel Navarro García (SPA) | Astana | 0:08:34 |
| 30 | Gregory Brenes Obando (CRC) | Burgos 2016 - Castilla y Leon | 0:09:08 |
| 31 |  Arnold Jeannesson (FRA) | Caisse d'Epargne | 0:09:53 |
| 32 | Mikel Landa Meana (SPA) | Orbea | 0:10:41 |
| 33 | Edgar Miguel Lemos Pinto (POR) | LA - Rota Dos Moveis | 0:11:22 |
| 34 | Francisco Perez Sanchez (SPA) | Caisse d'Epargne | 0:11:26 |
| 35 | Marcos Garcia Fernandez (SPA) | Xacobeo - Galicia | 0:11:36 |
| 36 | David García Da Pena (SPA) | Xacobeo - Galicia | 0:12:17 |
| 37 | José Ángel Gómez Marchante (SPA) | Andalucía - Cajasur | s.t. |
| 38 | Josep Jufre Pou (SPA) | Astana | 0:12:54 |
| 39 | Jon Izagirre Insausti (SPA) | Orbea | 0:13:26 |
| 40 | Gorazd Stangelj (SLO) | Astana | 0:13:57 |
| 41 | Pedro Merino Criado (SPA) | Footon - Servetto | 0:14:19 |
| 42 | Oscar Pereiro Sio (SPA) | Astana | 0:16:45 |
| 43 | Ángel Vicioso Arcos (SPA) | Andalucía - Cajasur | 0:16:48 |
| 44 | Rodrigo García Rena (SPA) | Xacobeo - Galicia | 0:17:55 |
| 45 | Arturo Mora Ortiz (SPA) | Caja Rural | 0:18:16 |
| 46 |  Fumiyuki Beppu (JPN) | Radio Shack | 0:18:17 |
| 47 | Markel Irizar Aranburu (SPA) | Radio Shack | 0:18:21 |
| 48 | Jose Luis Roldan Carmona (SPA) | Andalucía - Cajasur | 0:18:41 |
| 49 | Hugo Manuel Madeira Sabido (POR) | LA - Rota Dos Moveis | 0:18:45 |
| 50 | Virgilio Martins Dos Santos (POR) | LA - Rota Dos Moveis | 0:19:26 |
| 51 |  Edward King (USA) | Cervélo Testteam | 0:19:54 |
| 52 | Sergio Miguel Vieira Ribeiro (POR) | Barbot - Siper | 0:20:48 |
| 53 |  Michal Kwiatkowski (POL) | Caja Rural | 0:21:13 |
| 54 | Andoni Blazquez Berasarte (SPA) | Orbea | 0:22:04 |
| 55 | Iván Melero Coco (SPA) | Burgos 2016 - Castilla y Leon | 0:22:17 |
| 56 | Gonzalo Rabunal Rios (SPA) | Xacobeo - Galicia | 0:23:04 |
| 57 | Ricardo Garcia Ambroa (SPA) | Orbea | 0:23:44 |
| 58 | Hernâni Brôco (POR) | LA - Rota Dos Moveis | 0:23:49 |
| 59 | Rui Miguel Sousa Barbosa (POR) | Barbot - Siper | 0:24:42 |
| 60 | Steven Kruijswijk (NED) | Rabobank | 0:25:01 |
| 61 | Antonio Cabello Baena (SPA) | Andalucía - Cajasur | 0:25:07 |
| 62 | César Fonte (POR) | Barbot - Siper | 0:25:13 |
| 63 | Francisco Javier Aramendia Llorente (SPA) | Euskaltel - Euskadi | 0:25:31 |
| 64 | Stef Clement (NED) | Rabobank | 0:26:12 |
| 65 | Pablo Urtasun Perez (SPA) | Euskaltel - Euskadi | 0:26:48 |
| 66 | Miguel Minguez Ayala (SPA) | Euskaltel - Euskadi | 0:27:23 |
| 67 | Heldér Oliveira (POR) | Barbot - Siper | 0:27:29 |
| 68 |  Graeme Allen Brown (AUS) | Rabobank | 0:28:08 |
| 69 | Filipe Duarte Sousa Cardoso (POR) | LA - Rota Dos Moveis | 0:28:13 |
| 70 | Ruben Reig Conejero (SPA) | Caja Rural | 0:28:18 |
| 71 | Serafín Martínez Acevedo (SPA) | Xacobeo - Galicia | 0:28:25 |
| 72 | Benjamin Noval Gonzalez (SPA) | Astana | 0:29:05 |
| 73 | Marco Corti (ITA) | Footon - Servetto | s.t. |
| 74 |  Gert Steegmans (BEL) | Radio Shack | 0:29:25 |
| 75 | Dennis Van Winden (NED) | Rabobank | 0:29:36 |
| 76 | Pablo Lechuga Rodriguez (SPA) | Andalucía - Cajasur | 0:30:11 |
| 77 | Jesús Hernández Blázquez (SPA) | Astana | 0:30:13 |
| 78 |  Oleg Chuzhda (UKR) | Caja Rural | 0:30:21 |
| 79 | José Alberto Benitez Roman (SPA) | Footon - Servetto | 0:30:53 |
| 80 | David De La Fuente Rasilla (SPA) | Astana | 0:31:35 |
| 81 | Manuel Ortega Ocana (SPA) | Andalucía - Cajasur | 0:31:39 |
| 82 | David Gutierrez Gutierrez (SPA) | Footon - Servetto | 0:31:41 |
| 83 | Francisco Manuel Anton Marin (SPA) | Burgos 2016 - Castilla y Leon | 0:32:19 |
| 84 | Lluís Guillermo Mas Bonet (SPA) | Burgos 2016 - Castilla y Leon | 0:32:31 |
| 85 | Enrique Mata Cabello (SPA) | Footon - Servetto | 0:32:50 |
| 86 | Oscar Grau Jorda (SPA) | Burgos 2016 - Castilla y Leon | 0:32:54 |
| 87 | Ruben Calvo Gomez (SPA) | LA - Rota Dos Moveis | 0:32:59 |
| 88 | Jorge Azanza Soto (SPA) | Euskaltel - Euskadi | 0:33:47 |
| 89 | Theo Bos (NED) | Cervélo Testteam | 0:33:50 |
| 90 | Joaquin Ortega Gascon (SPA) | Barbot - Siper | 0:34:36 |
| 91 |  Ignatas Konovalovas (LTU) | Cervélo Testteam | 0:35:39 |
| 92 | David Francisco Delgado (SPA) | Burgos 2016 - Castilla y Leon | 0:36:13 |
| 93 | Benat Urain Epelde (SPA) | Orbea | 0:36:43 |
| 94 | Xabier Zabalo Imaz (SPA) | Orbea | 0:37:07 |
| 95 | José Vicente Toribio Alcolea (SPA) | Andalucía - Cajasur | 0:37:47 |
| 96 | Jason McCartney (USA) | Radio Shack | 0:39:31 |
| 97 | Iban Mayoz Etxebarria (SPA) | Footon - Servetto | 0:39:56 |
| 98 | Nicolas Jonathan Castroviejo (SPA) | Euskaltel - Euskadi | 0:40:42 |
| 99 | Mikel Ilundain Tirapu (SPA) | Orbea | 0:41:22 |
| 100 |  Tomas Faiers (GBR) | Footon - Servetto | 0:41:28 |
| 101 | Joaquín Novoa Mendez (SPA) | Cervélo Testteam | 0:42:18 |
| 102 | Adrian Saez De Arregi Egurrola (SPA) | Orbea | 0:42:39 |
| 103 | Oscar Pujol Muñoz (SPA) | Cervélo Testteam | 0:44:39 |
| 104 | João Correia (POR) | Cervélo Testteam | 0:47:56 |
